Effect of glycosylated and non-glycosylated prolactin on the proliferation of normal human lymphocytes
1997
Abstract
We evaluated the effects of 2.5x10(-10) M or 5x10(-10) M concentrations of human pituitary prolactin (pPRL), human recombinant non-glycosylated (NG-PRL) and glycosylated (GL-PRL) prolactin on the proliferation of normal human lymphocytes with or without coactivation by interleukin-2 (IL-2). None of the PRL forms alone affected the lymphocyte proliferation in a serum-free medium, however, the stimulatory activity of IL-2 was significantly potentiated with all 3 PRL variants.
Since the 5x10(-10) M concentrations of individual PRLs exerted the same effects, this result suggests that GL-PRL in primary lymphocyte culture is not a less mitogenic form, if sufficient amounts of IL-2 are available.