The optimalisation and validation of HPLC method for determination of triamcinolone acetonide in topical pharmaceutical product
Abstract
A novel reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of active component triamcinolone acetonide, its degradation product triamcinolone and two preservatives presented in the cream - methylparaben and propylparaben, using estradiole as an internal standard.