Possibility of the affecting of secondary metabolites production in Silybum marianum culture in vitro
Abstract
The effect of 6, 12, 24, 48, 72 and 168 hours influence by three concentrations of the abiotic elicitor (3 jodo 4 methylfenyl)amide 5-methylpyrazine-2-carboxylic acid on the flavonolignans production in Silybum marianum callus and suspension culture was monitored in this study. The maximum content of flavonolignans (0.03 %) in callus culture was demostrated after 24 hours of elicitation (c1 = 2.83•10-3 mol/l).
The maximum content of flavonolignans (0.04 %) in suspension culture was demonstrated after 48 hours of elicitation (c3 = 2.83•10-5 mol/l).