Abstrakt
Ellipticine (5,11-demethyl-6H-pyridol(4,3-b) carbazole) and its derivates belong to prototype compounds acting predominantly by DNA-damaging mechanisms and serving as cytotoxic agents. The aim of this work was to study the intercalation of the ellipticine into DNA.
For this purpose we utilized adsorptive transfer stripping technique (AdTS) coupled with square wave voltammetry (SWV). Primarily, we investigated the influence of ellipticine on genomic DNA electrochemical signals.
Decrease in redox signal of cytosine/adenine (at -1.4 V) was observed as a result of ellipticine intercalation into DNA. The decrease was proportional to increasing concentration of ellipticine.
In additional experiments we investigated the changes in the reduction signal of cytosine/adenine (CA) in a genomic dsDNA, dsDNA treated with ellipticine in vitro and dsDNA isolated from UKF-NB-3 neuroblastoma cells treated with 1 mu M ellipticine. In the mentioned cases, the changes were determined.