Beta defensins 2 and 3 in mucosa of nasal polyps in patients with chronic rhinosinusitis and nasal polyps with a cultivation finding of staphylococcus aureus on nasal mucosa
Abstract
Chronic sinusitis with nasal polyps is an inflammatory disease, where bacterial infection can participate in etiopathogenesis. Human beta defensins (HBD) are antimicrobial peptides, which represent an important component of inborn mucous immunity.
Objective: The study investigated whether HBD 2 and 3 are demonstrable at higher levels in nasal polyps as compared with healthy nasal mucosa. Method: Before sampling of the nasal polyps, cultivation examination of the secrets from nasal cavity was performed.
Samples of healthy nasal mucosa (n = 11) and nasal polyps (n = 49) were processed for light microscopy by fixation in 4% formaldehyde for embedding in paraffin, a portion of the material was frozen in liquid nitrogen at -80°C. HBD 2 and 3 were detected in paraffin and cryostat sections by immunohistochemical methods by means of three-stage immunoperoxidase technique.
Results: Staphylococcus aureus was the most often cultivated pathogen in patients with nasal polyps. In clinically healthy nasal mucosa the presence of HBD-2 was determined in individual cells of glandular ducts and in ciliary cells.
In the polyp material the presence of HBD-2 was demonstrated at higher amounts than in clinically healthy nasal mucosa. HBD-2 was demonstrated in ciliary cells and cellular cytoplasm of the glandular ducts.
HBD-3 was demonstrated in ciliary cells of surface epithelium as well as in the cells of glandular ducts in the polyps. The reaction product of proved HBD-2 occurred at extremely high amounts in the cell cytoplasm of surface epithelium as well as in cells of gland ducts in the polyp samples with demonstrated presence of Staphylococcus aureus.
Conclusion: It has become obvious that defensins HBD 2 and 3 occurred more often in the tissue of nasal polyps than in healthy nasal mucosa. HBD 3 was demonstrated at extremely high amount in samples of polyps with demonstrated presence of Staphylococcus aureus.