S-Adenosylmethionine does not Reduce Ischaemia-Reperfusion Injury to a Marginal Liver Graft in an In vivo Experiment
Abstract
Background/Aims: There are a limited number of appropriate cadaver liver donors. One possible solution is the use of marginal liver grafts from cadaver donors for liver transplantation.
Methodology: Rats with liver steatosis were divided into four containing seven animals steatotic liver grafts; +S-adenosylmethionine; I-b: steatotic liver grafts were transplanted; no S-adenosylmethionine; II-a: normal liver grafts, +S-adenosylmethionine; II-b: normal liver grafts. Blood samples were taken at days -1, 3 and 14.
Results: ALT values at day 14: 1.75 +/- 1.10 mu kat/L (in group I-a), 1.91 +/- 1.41 mu kat/L (in group I-b), 2.13 +/- 1.85 mu kat/L (in group II-a) and 2.08 +/- 1.35 mu kat/L (in group II-b). There were no significant differences between these values.
GSH values at day 14 post-transplantation were: 44.90 +/- 8.61 mu M/ mg (in group I-a), 43.82 +/- 8.58 mu M/mg (in group I-b), 41.65 +/- 4.87 mu M/mg (in group II-a) and 42.71 +/- 4.17 mu M/ mg (in control group II-b). Conclusions: Our study did not demonstrate the positive effect of S-adenosylmethionine on ischaemia-reperfusion injury during liver transplantation in rats.