Voltammetry Assay for Assessment of Oxidative Stress linked Pathologies in Brain Tumor suffered Childhood Patients
Abstract
Oxidative stress plays an important role in cancers due to several reasons including metabolism disorder and hypoxia in tissue near the cancer. Numerous researchers have reported participation of oxidative stress in the cancer pathology.
However, the mechanism is not well understood. In this study, we investigated oxidative stress development and depletion of antioxidants in blood samples from medulloblastoma, neuroblastoma, glioblastoma and central nervous system cancer suffered childhood patients.
The blood samples were analyzed using square wave voltammetry (SWV), dimethyl-4-phenylenediamine method (DMPD), 2'-azinobis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), free radical method (FR), ferric reducing antioxidant power (FRAP), reduced and oxidized glutathione, and metallothionein. We proved significant correlation between the markers.
The findings demonstrate crucial role of oxidative stress and antioxidants levels regulation in the cancer suffered patients.