Transfer of genomics information to flow cytometry: expression of CD27 and CD44 discriminates subtypes of acute lymphoblastic leukemia
2005
Abstract
Expression profiling studies have provided data on an unprecedented number of genes that are expressed in malignant cells. The critical number of genes which can predict a particular genotype is a matter of discussion.
Downing pointed out that as few as 20 genes may be necessary for an accurate prediction of subsets and prognosis; these genes should be specifically studied, perhaps by methods like reverse transcription polymerase chain reaction (RT-PCR) or flow cytometry (FC) (Downing in Carroll et al5). FC, which shows the expression of molecules in mutual context on individual cells, appears optimal for such analysis.