Preparation of Base-Modified DNA by Enzymatic Incorporation of Modified Nucleoside Triphosphates

Abstract

Synthesis of base-modified oligonucleotides and DNA by polymerase incorporations of modified nucleoside triphosphates (dNTPs) is summarized. The cross-coupling synthesis of modified dNTPs, methods of their enzymatic incorporations, as well as their applications in bioanalysis and chemical biology are discussed.

Keywords

• DNA
• nukleotidy
• cross-coupling
• polymerasy