Abstrakt
tThe effect of different concentrations of CTAB (in the range of 0.2 mMMINUS SIGN 2mM, i.e., including its criticalmicelle concentration) on viability of selected human cells (osteoblasts and keratinocytes) was stud-ied by a variety of methods (immunocytochemical and biochemical), testing the cell viability and themetabolism, to get a complex overview. All of the used methods confirmed the cytotoxic effect of CTAB,which could, however, be suppressed by the presence of hyaluronan (molecular weight 806 kDa, in theconcentration of 1 g/l) in the case of the lowest CTAB concentration used (0.2 mM) when the fetal bovineserum was also present in the cultivation medium.
Thus, it could be concluded that hyaluronan can beused as successful protector of specific cell types against cytotoxic CTAB at low concentrations.