Metabolic pathways of anthelmintic drug monepantel in sheep and in its parasite (Haemonchus contortus)
Abstract
The aim of our study was to find out metabolic pathways of MOP in sheep in vivo and in its parasite Haemonchus contortus ex vivo. MOP biotransformation in two H. contortus strains with different sensitivity to anthelmintics was also compared.
Ultrahigh-performance liquid chromatography with tandem mass spectrometry (UHPLC/MS/MS) technique is used for the identification of MOP metabolites in ovine urine, faeces and in nematodes. MOP biotransformation study in sheep in vivo led to the identification of 13 MOP metabolites, 7 of them have not been described previously in in vitro study.
The study of MOP biotransformation in H. contorus ex vivo reveals 4 MOP metabolites. The nitrile hydrolysis as a new biotransformation pathway in helminths ex vivo is reported here for the first time.