Abstract
The aim of this Thesis is to demonstrate the application of the tools of expression proteomics in solving diverse challenges in biomedicine. We employed various proteomic approaches and tools for studying molecular mechanisms of human diseases using pacient biological samples, or a model organism and a cell culture.
We were conducting three different research projects, namely: A quest for potencial molecular targets for selective elimination of TRAIL-resistant mantle cell lymphoma cells; Investigation of molecular mechanisms of heart failure using a rat model of the disease induced by volume overload; and Searching for diagnostically usable serum biomarkers of ovarian cancer. Results of our three projects are discovery of molecular "weakness" of resistant cells which has a potencial as a therapeutic target for the selective elimination of such cells, suggestion of two highly potential therapeutic targets for treatment of heart failure and identification of a new potential biomarker of ovarian cancer.