Abstract
Isolated porcine pepsinogen A was used for the preparation of rabbit (IgG) and chicken (IgY) anti-pepsinogen A antibodies. Immunochemical properties of both immunoglobulin fractions were compared.
The rabbit anti-serum was further purified using immobilized porcine pepsinogen A on magnetic cellulose beads and the resulting IgG fraction proved to be applicable for the separation and the determination of porcine pepsinogen A. In contrary, antibodies prepared from chicken eggs by the same way have been found not suitable for that purpose.
Unexpectedly, the pre-immune fraction of IgY showed reactivity against porcine pepsinogen A and the affinity separation of IgY on the used immunomagnetic carrier from immunized hen did not concentrate anti-pepsinogen A antibodies.