The use of formalin-fixed, paraffin-embedded lymph node samples for the detection of minimal residual disease in mantle cell lymphoma

Abstract

There is a growing evidence regarding the usefulness of minimal residual disease (MRD) monitoring in mantle cell lymphoma (MCL) (Pott, 2011). MRD assessment in MCL can be performed either at the gene expression level using CCND1- IGH transcript (Pott, 2011), via CCND1 overexpression (Brizova et al, 2008) or at the DNA level.

Quantitative polymerase chain reaction (qPCR) for clone-specific immunoglobulin (IGH) gene rearrangement is currently the most sensitive and robust tool for MRD detection and has been thoroughly standardized for use in acute lymphoblastic leukaemia (ALL) (van der Velden et al, 2007).

Keywords

• formalin-fixed paraffin-embedded
• immunoglobulin heavy chain
• mantle cell lymphoma
• minimal residual disease
• quantitative PCR