Abstrakt
Purpose: Acute gastroenteritis is one of the most common diseases in humans worldwide and represents a significant cause of mortality and morbidity. The majority of cases are of viral aetiology, evidence for which has been increasing in the past decade.
Several studies on the prevalence in European countries of viral aetiology of gastroenteritis have been published in the last decade, but none from the Czech Republic. Material and methods: In total 107 faeces samples obtained from patients hospitalised in the University Hospital in Hradec Králové were examined by immunochromatographic tests using ROTA-ADENO Card Rapid-Viditest (VIDIA, Czech Republic) and RidaQuick Norovirus (R-Biopharm, Germany), and by an in-house Real-time PCR panel.
Results: Overall findings of viruses detected by PCR in the tested faeces samples were: rotaviruses in 29.9 %, noroviruses in 14.0 % and adenoviruses in 5.0 %. Immunochromatographic antigen detection performed at lower sensitivity compared with PCR: rotaviruses in 28.0 %, noroviruses in 4.7 % and adenoviruses in 2.0 %.
Our findings demonstrate even lower sensitivity of the used immunochromatographic tests compared with manufacturers' data. Conclusion: Our study has revealed limitations in immunochromatographic tests, especially in their sensitivity and the necessity for using another confirmatory method.
We have set up real-time PCR in routine diagnosis of viral gastroenteritis in our hospital.