We present a measurement of the electric charge of top quarks using t (t) over bar events produced in p (p) over bar collisions at the Tevatron. The analysis is based on fully reconstructed t (t) over bar pairs in lepton + jets final states.
Using data corresponding to 5.3 fb(-1) of integrated luminosity, we exclude the hypothesis that the top quark has a charge of Q = -4/3e at a significance greater than 5 standard deviations. We also place an upper limit of 0.46 on the fraction of such quarks that can be present in an admixture with the standard model top quarks (Q = +2/3e) at a 95% confidence level.