Abstract
Considerable body of evidence shows bacterial community and especially its anaerobic compartment residing periodontal environment as the causative agents of periodontal diseases. The cultivation of these fastidious microorganisms is very demanding and many of them are not cultivable at all.
Microbiological detection and diagnostic techniques so far employed in periodontal microbiology detected several keystone bacteria as putative pathogens worth to be used as markers of progression risk in periodontal pathology. Aim: The aim of this study was test the relevance of the 454 pyrosequencing technique for the simplified identification of the broad spectrum of periodontal microbiome including keystone bacteria Tannerella forsynthia, Porphyromonas gingivalis, Treponema denticola, etc. for the prediction of risk of follow-up periodontal breakdown.
Material and methods: 15 patients with adult periodontitis (AP) cared in periodontal maintenance regime, 15 patients with AP without previous therapy and 18 periodontally healthy individuals were involved in the study. All subjects were sampled for the contents of periodontal pockets/gingival sulci and the material was processed in the 454 pyrosequencing technology.
An attempt has been made to define, based on results of individual samples analyses, the risk bacterial pattern (RBP). Results: Both groups of periodontally involved patients showed the RBP while in periodontally healthy individuals this RBP was found only occasionally.
Conclusion: Screening periodontal bacterial community by means of the 454 pyrosequencing technology can thus provide significant data for the risk assessment in patients scheduled for surgery of heart or big vessels.