Publication at Faculty of Science |
2014
Abstract
New fluorescent nucleosides and nucleoside triphosphate (dNTPs) analogs bearing the F-Bodipy fluorophore linked through a short, flexible nonconjugate tether were synthesized. The Bodipy-labeled dNTPs were substrates for several DNA polymerases which incorporated them into DNA in primer extension, nicking enzyme amplification reaction, and polymerase chain reaction.
The fluorescence of F-Bodipy is not quenched upon incorporation in DNA and can be detected both in solutions and on gels.