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Structural Characterization of Phosducin and its Complex with the 14-3-3 Protein

Publikace na Přírodovědecká fakulta, Matematicko-fyzikální fakulta |
2015

Tento text není v aktuálním jazyce dostupný. Zobrazuje se verze "en".Abstrakt

Background: Phosducin is a conserved regulatory phosphoprotein involved in phototransduction whose function is regulated in a 14-3-3-dependent manner. Results: The 14-3-3 protein binding affects the structure and the accessibility of several regions within both domains of phosphorylated phosducin.

Conclusion: The 14-3-3 protein sterically occludes the whole G(t) binding interface of phosducin. Significance: Mechanistic explanation is given for the 14-3-3-dependent inhibition of phosducin function.

Phosducin (Pdc), a highly conserved phosphoprotein involved in the regulation of retinal phototransduction cascade, transcriptional control, and modulation of blood pressure, is controlled in a phosphorylation-dependent manner, including the binding to the 14-3-3 protein. However, the molecular mechanism of this regulation is largely unknown.

Here, the solution structure of Pdc and its interaction with the 14-3-3 protein were investigated using small angle x-ray scattering, time-resolved fluorescence spectroscopy, and hydrogen-deuterium exchange coupled to mass spectrometry. The 14-3-3 protein dimer interacts with Pdc using surfaces both inside and outside its central channel.

The N-terminal domain of Pdc, where both phosphorylation sites and the 14-3-3-binding motifs are located, is an intrinsically disordered protein that reduces its flexibility in several regions without undergoing dramatic disorder-to-order transition upon binding to 14-3-3. Our data also indicate that the C-terminal domain of Pdc interacts with the outside surface of the 14-3-3 dimer through the region involved in G(t) binding.

In conclusion, we show that the 14-3-3 protein interacts with and sterically occludes both the N- and C-terminal G(t) binding interfaces of phosphorylated Pdc, thus providing a mechanistic explanation for the 14-3-3-dependent inhibition of Pdc function.