Abstrakt
This study presents an applicability of real-time microradiography to biological objects, namely to horse chestnut leafminer, Cameraria ohridella (Insecta: Lepidoptera, Gracillariidae) and following image processing focusing on image segmentation and object recognition. The microradiography of insects (such as horse chestnut leafminer) provides a non-invasive imaging that leaves the organisms alive.
The imaging requires a high spatial resolution (micrometer scale) radiographic system. Our radiographic system consists of a micro-focus X-ray tube and two types of detectors.
The first is a charge integrating detector (Hamamatsu flat panel), the second is a pixel semiconductor detector (Medipix2 detector). The latter allows detection of single quantum photon of ionizing radiation.
We obtained numerous horse chestnuts leafminer pupae in several microradiography images easy recognizable in automatic mode using the image processing methods. We implemented an algorithm that is able to count a number of dead and alive pupae in images.
The algorithm was based on two methods: 1) noise reduction using mathematical morphology filters, 2) Canny edge detection. The accuracy of the algorithm is higher for the Medipix2 (average recall for detection of alive pupae = 0 : 99, average recall for detection of dead pupae = 0 : 83), than for the flat panel (average recall for detection of alive pupae = 0 : 99, average recall for detection of dead pupae = 0 : 77).
Therefore, we conclude that Medipix2 has lower noise and better displays contours (edges) of biological objects. Our method allows automatic selection and calculation of dead and alive chestnut leafminer pupae.
It leads to faster monitoring of the population of one of the world's important insect pest.