Rapid Determination of alpha-Hederin and Hederacoside C in Extracts of Hedera helix Leaves Available in the Czech Republic and Poland
Abstract
Leaf extracts of Hedera helix L. are widely used in the treatment of upper respiratory diseases. The saponins alpha-hederin and hederacoside C are considered to be the main compounds responsible for the biological activity. alpha-Hederin and hederacoside C were determined in H. helix leaf extracts using a fast, simple and validated HPLC method.
An XTerra MS C18 column and mobile phase composed of 10 mM ammonium acetate at pH 8.5 (adjusted with triethylamine) and acetonitrile were used for the chromatography at 1.2 ml min(-1). The column was kept at 30 degrees C.
Detection was performed at 220 nm. An approach utilizing a basic pH of the aqueous part of the mobile phase enabled analysis in 5 minutes in isocratic mode.
The method was validated and used for the quality control of H. helix leaf ethanolic extracts.