We have presented a protocol for porcine liver vascular bed casting based on corrosion specimens prepared using Biodur E20 epoxy resin (Biodur Products, Heidelberg, Germany). Biodur E20 permeates throughout the entire microvascular bed and offers sufficient radioopacity, as well as minimum fragility.
Macro- and micro-CT scans enabled 3D reconstruction of the entire vascular bed. The micro-CT images enabled stereological assessment.
In combination with SEM, corrosion casting increases the possibility of morphometric analysis of a vascular network in health and disease.