The study was carried out at six sites in the Jizerskehory Mts. in the north of the Czech Republic. At all these sites, ranging in altitude between 460 and 962 m a. s. l., and during the period from June to September in 2008, O-3 concentrations and environmental parameters important for accumulated stomatal O-3 flux (AF(st)) into Fagus sylvatica leaves were measured.
At five sites, visible injury on Fagus sylvatica L. juvenile tree leaves was observed. A combination of actual O-3 levels in the Jizerkehory Mts. and environmental conditions, though relative air humidity and air temperature significantly limited stomatal conductance, has been sufficient enough to cause O-3 uptake exceeding the critical level (CL) for forest ecosystems.
The AFst values ranged between 13.4 and 22.3 mmol O-3 m(-2). The CL for the accumulated stomatal flux of O-3 above a flux threshold 1.6 nmol m(-2) s(-1) (AF(st)1.6) was exceeded at all sites from ca 45 to 270 % (160 % on average).
The CL of 5 ppm h(-1) for AOT40 (accumulated O-3 exposure above threshold of 40 ppb) was exceeded at four sites. The relationship between visible injury on O-3 indices was found.
The conclusions based on AOT40 and AF(St) are not the same. AF(St) has been determined as better predictor of visible injury than AOT40.