Multifunctional Amphiphilic Nanoparticles Featuring (Bio)Degradable Core and Dual-Responsive Shell as Biomedical Platforms for Controlled Release
Abstract
Multifunctional polymeric platforms combining (bio)degradable and biocompatible, temperature and pH-sensitive entities hold great promise as nanocarriers for targeted drug and gene delivery, and tissue engineering. In this work, preparation and characterization of surfactant-free polyester nanoparticles (NPs) from biobased polyesters poly(butylene sebacate) (PBSE) and poly(butylene sebacate-co-butylene dilinoleate)s (PBSE/PBDL) using nanoprecipitation is reported.
This strategy leads to spherical nanosized particles with sizes narrowly distributed in a range of 30-200 nm which is appropriate for internalization by a variety of cells. The effect of molecular parameters and type of solvent used in the nanoprecipitation protocol on the size and shape of produced polyester nanocolloids and their in vitro degradation in PBS solution at 37 degrees C is elucidated by quasi-elastic light scattering (QELS), atomic force microscopy (AFM), transmission electron microscopy (TEM), and size-exclusion chromatography (SEC) techniques.
A dense cationic brush layer (approximate to 20 nm) of stimuli-responsive and biocompatible poly(2-dimethylaminoethyl methacrylate-co-acrylonitrile) is grafted on the surface of PBSE/PBDL NPs through grafting onto (arm first) coupling chemistry.