Publication at Faculty of Mathematics and Physics |
2015
Abstract
The invention of light microscope belongs to the most fundamental contributions ever made to the advancement of biology. This imaging technique played also an important role in material science and other disciplines, as well as in many practical applications.
Before the end of the 19th century, Lord Rayleigh and Ernst Abbe recognized that the resolution limit of optical microscopes is about half the wavelength of the light used. In the past two decades, however, several revolutionary methods occurred that improved the resolution of optical microscopes to such an extent that, instead of the microscopy, we can now talk about optical nanoscopy.