Cytokine production by SaOS-2 and mononuclear cells cultivated on alginate titanium surfaces doped with calcium phosphates
Abstrakt
The immune response of the host tissue to tested biomaterial predicts implantation therapy success. Connective tissue cell line SaOS-2 and mononuclear cells isolated from ""buffy coats"" testing is determination of overall immune response to the implanted biomaterial.
Alginate hydrogel coatings doped with inorganic hydroxyapatite (Ti/Alg/HAP) or beta-tricalcium phosphate (Ti/Alg/TCP) nanoparticles on Titanium grade 2 were prepared. Both cell types mentioned above were used to evaluate the cytokine production when cultivated on the proposed coatings.
The pristine Ti and alginate (Alg) served as control surfaces. Cytokine production was assessed by the multiplex proteomic analysis of 40 cytokines.
Mononuclear cells testing revealed differences between control (Ti, Ti/Alg) and alginate hydrogel surfaces doped with calcium phosphates (Ti/Alg/TCP and Ti/Alg/HAP). Cytokine production declines from highest found on mononuclear cells treated by Ti/Alg/HAP through control Ti and Ti/Alg/TCP surface to control Ti/Alg surface with lowest cytokine production.
Mononuclear cells produced mostly IL-6 and IL-8. No significant differences between Ti/Alg/TCP and Ti/Alg/HAP were found in production of cytokines by cell line SaOS-2.
Cell line SaOS-2 produced wide spectrum of cytokines, but the production was low, with exception of TIMP-2. Comparison of cytokines produced by cell line SaOS-2 cells and mononuclear cells from ""buffy coat"" showed much better potential of mononuclear cells to show the differences between the monitored materials.
The immune response of mononuclear cells showed differences between tested materials, whereas SaOS-2 cells weren't sufficiently sensitive. Therefore, besides SaOS-2 cells, mononuclear cells should be also considered for in vitro evaluation of overall immune response induced by presence of the implant.