Detection of helicobacter pylori and human papillomavirus in peroperative tissue biopsies collected from malignancies in oropharyngeal area
Abstract
We investigated 70 tissue specimens collected during cancer surgery for detection of bacterial DNA of Helicobacter pylori and viral DNA of High risk HPV (groups A9, A7 and A5/6) using rt-PCR. Results: Helicobacter pylori DNA was detected in 60 samples (85.7%), while DNA of HPV only in 42 (60%).
HPV-16 as proposed cancer inductor was detected in 34 samples (48.5%) only. No DNA of respective agents was found in 7 samples (10%).
There were 21 Helicobacter sole pathogen detections compared with only 3 of HPV. Helicobacter pylori is a long-term resident in oropharynx and tonsils and. may influence functions of immune system, so that a newly entering contributor could switch-on the process resulting in cancer development.
This supports high incidence of common detection of HPV and Helicobacter pylori in 39 samples (55.7%).