Abstract
In this study, a cellular resistance to selected antirheumatics FAF-12 and FAF-14 has been tested, namely their abilities to interact with ABC drug efflux transporters ABCB1, ABCG2 and ABCC2 have been evaluated. These membrane transporters exhibit considerable efflux activity thereby influencing pharmacokinetic behavior of drugs, moreover, they are responsible for the phenomenon of multidrug resistance.
XTT proliferation method in MDCKII-ABCB1, MDCKII-ABCG2 a MDCKII-ABCC2 cells has been employed for study of the role of ABC transporters in the cell resistance to studied antirheumatics. Based on our results, it is feasible to presume that ABCB1 and ABCG2 can give a rise to resistance toward both FAF-12 and FAF-14 whereas ABCC2 does not take a part in the resistence to any of the studied drugs.