Abstract
The inflation-extension test was performed in order to obtain the mechanical response (stress-strain curves) of vena saphena magna (usually used for coronary artery bypass graft surgery). Tubular samples of the vein were inflated four times up to the pressure approx. 4 kPa (vein pressure) and then four times up to approx. 16 kPa (systolic pressure).
The experiments were recorded by the CCD camera. The longitudinal and circumferential deformations of the tube were evaluated using the edge detection method.
The experimental data were fitted by anisotropic, nonlinear, constitutive model in order to obtain model parameters, especially the parameter which can be explain as collagen fibres orientation approximation. This parameter was then compared with the findings from histology.
The histology analyses based on label-free imaging were performed additionally to the mechanical testing. Collagen (most important load-bearing component of the vein wall) was visualized using second harmonic generation imaging (SHG, excitation at 860 nm by a tunable IR pulse laser, detection at 430+-10 nm).
This method enabled us to observe collagen through the vein wall. It was found that the collagen fibres are helically aligned within the vein at an angle 37+-6o measured from circumferential axis.
The results show a high correlation between the arrangement of collagen obtained from histology and constitutive model.