Publication at First Faculty of Medicine |
2016
Abstract
Objective: The aim of this study was the verification of the applicability of NPHS2/SYNPO ratio for diagnosis of focal segmental glomerulosclerosis (FSGS) and minimal change disease (MCD) in Czech patients and identification of new markers for distinguishing between MCD and FSGS. Material and methods: Our study was performed on 24 samples from patients with FSGS and MCD.
Gene expressions were determined using relative quantitative real-time PCR and TaqMan(R) Array Micro Fluidic Cards and normalized to the reference gene GAPDH. Results and conclusion: Our results did not find any significant difference in the podocin/synaptopodin ratio and in any other studied genes between FSGS and MCD.