Changes in Paraoxonase 1 activity and concentration of conjugated dienes in connection with number of metabolic syndrome components
Abstract
Objective: Paraoxonase 1 is an antioxidant enzyme with a variety of physiological roles - one of them is the inhibition of LDL (low-density lipoprotein) lipid peroxidation and inactivation of LDL-derived oxidized phospholipids. The aim of this study was to investigate the PON1 activity and levels of conjugated dienes in precipitated LDL (CD/LDL) in subjects, who fulfill different numbers of metabolic syndrome (MetS) criteria.
Design: Cross-sectional study Settings: 4th Department of Internal Medicine, 1st Faculty of Medicine, Charles University and General University Hospital Prague Material and methods: The population under study consisted of 354 Caucasian subjects (188 females/166 males) divided into 6 groups according to the number of presented components of metabolic syndrome. All groups were age matched.
The activity of paraoxonase 1 and concentration of conjugated dienes in precipitated LDL (CD/LDL) were both assessed spectrophotometrically. Results: The activity of PON1 was significantly decreased in subjects with all 5 components of MetS in comparison with those with 0 to 3 components of MetS (p < 0.05).
The concentrations of CD/LDL were increased in subjects with 4 or 5 components of MetS compared to subjects with 0-3 components of MetS (p < 0.001). Conclusion: As was shown in this study, the levels of PON1 are extensively affected by the concentration of HDL-C and ApoA1.
PON1 activity is depressed and CD/LDL levels are increased mainly in subjects who fulfill all five criteria of MetS.