Abstract
In early developmental stages of brain, many maturational processes on molecular, cellular and organ level take place. Although many years of research were dedicated to this topic, the clarification of numerous mechanisms and their roles in later development is still lacking.
Combination of microdialysis (MD) and high performance liquid chromatography combined with mass spectrometry (HPLC/MS) represents a unique possibility for the monitoring of tissue biochemistry and metabolomic mapping in immature brain in almost real-time condition. Hence, it has a great potential to broaden our knowledge in physiology and pathophysiology of immature brain and can help to develop effective treatment of many infant diseases.