Carrier Molecules and Extraction of Circulating Tumor DNA for Next Generation Sequencing in Colorectal Cancer

Abstract

The aims of the study were: i) to compare circulating tumor DNA (ctDNA) yields obtained by different manual extraction procedures, ii) to evaluate the addition of various carrier molecules into the plasma to improve ctDNA extraction recovery, and iii) to use next generation sequencing technology to analyze mutations in ctDNA from patients with metastatic colorectal cancer.

Keywords

• Carrier
• Extraction
• Circulating tumor DNA
• Next generation sequencing
• Real-time PCR