Determination of selected components of human urine by electrophoresis in a short capillary with pressure-assisted sampling
Abstract
A hydrodynamic sample introduction method has been proposed for electrophoresis in short capillary. The separation electrolyte flushes sample from the loop of a six-way sampling valve and carries it to the injection end of the capillary.
At the time when the sample zone reaches the capillary, a short pressure impulse is generated in the electrolyte stream, which provides injection of the sample into the capillary. Then the electrolyte flow is stopped and the separation voltage is turned on.
The method was used for the determination of selected components in urine. The determination was performed in a capillary with an overall length of 10.5 cm and two base electrolytes was tested.
A, dual contactless conductivity and UV spectrometric, detection was used.