Efficient generation of arsanes coupled with HPLC or with cryotrapping for speciation analysis of arsenic
Abstract
The aim of this work was to find conditions of hydride generation to generate four arsenic species (arsenite, arsenate, monomethylarsonate, dimethylarsinate) with the same efficiency in a flow injection and batch mode. In the next step such generators and optimized conditions of hydride generation were employed for speciation analysis of arsenic.
In the first case arsenic species were separated by HPLC and the sample injection valve was substituted with the HPLC outlet. In the second case the batch generator was connected with a cryotrapp that separated generated arsanes according to their different boiling points.
Due to same generation efficiency (and thus sensitivity) single species standardization could be used in the both approaches. This has been successfully demonstrated on urine sample with postcolumn hydride generation so far.
The preliminary results with a cryotrappare also very promising.