Abstract
The alloplastic materials currently used for protective surface layers on implants were tested in vitro under microbiological laboratory conditions by contamination with microbial agents most frequently found in deep infection of total joint replacements. The objective was to find out how the resistance to bacterial colonisation was related to different surface finishes.Each of 14 samples of alloplastic material currently used in the manufacture of orthopaedic implants was inoculated with each of the group of microorganisms most frequently infecting joint replacements; these were Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Enterococcus faecalis and Escherichia coli.
At 24 hours of incubation, biofilms produced on sample surfaces were collected, stained with crystalline violet and assessed by spectrophotometry. The average value of biofilm absorbances (AV595) for the group of microorganism tested was taken as a basic characteristic of each material sample indicating its sensitivity to bacterial.Of the metal materials with smooth surface finish, Vitalium (AV595, 0.368) showed the lowest affinity to microbial colonisation; next was titanium (AV595, 0.459) and steel (AV595, 0.505).
A significant increase in sensitivity to bacterial colonisation was recorded in all types of surface finish of steel (AV595, 0.571) and in titanium alloy with a rough surface texture (AV595, 0.737 to 1.676); p < 0.05. Porous titanium surfaces significantly increased material affinity to colonisation.