Investigation of polyaniline-coated silica gel as a stationary phase for separations in different modes of capillary liquid chromatography
2016
Abstract
Separation potential of a home-made stationary phase based on polyaniline-coated silica gel particles was investigated in different chromatographic modes of capillary liquid chromatography. Three sets of testing solutes comprised L-tryptophan and structural analogues, positional isomers of aminoacetophenone, and caffeine and its demethylated analogues.
The stationary phase exhibits the mixed-mode retention mechanism, thus slightly hydrophilic compounds can be retained in RP mode and vice versa. We successfully separated caffeine, theobromine and theophylline in NP, HILIC, and RP modes.
All positional isomers of aminoacetophenone were separated for the first time in NP and RP modes.