Determination of rhenium complexes with 1,2-dihydroxybenzene and 1,2,3-trihydroxy-benzene by CZE compared with HPLC-UV/MS
Abstrakt
Two analytical methods, HPLC and CZE, have been successfully used for determination and separation of reaction mixtures containing rhenium complexes, a potentially important group in medicine, prepared by a reaction of tetrabutylammonium tetrachlorooxo-rhenate as rhenium precursor and complex forming ligands 1,2,3-trihydroxybenzene and 1,2-dihydroxybenzene. Borate buffers in the range of pH6.0-11.5were tested as background electrolytes, all of them provide complete resolution of all components of a reaction mixtures within 10 min.
Effective electrophoretic mobilities of separated components were calculated and relevant graphs were designed. Optimal conditions for HPLC-UV/MS separation of reaction mixture components have been successfully discovered for the Agilent Zorbax SAX column, fully resolved peaks were identified by amassspectrometry with electrospray ionization.