Voltammetric determination of homovanillic acid at boron doped diamond electrode for hollow fibre microextraction
Abstrakt
Presented work deals with hollow-fibre assisted liquid/liquid/liquid microextraction (HF-LPME) and subsequent voltammetric detection of clinical biomarker homovanillic acid (HVA). Three main areas are discussed: hollow fibre assisted extraction of HVA, voltammetric detection of HVA under basic conditions, and sensing in very small volumes.
Using HF-PLME, HVA was extracted with 1-octanol as a supported liquid membrane with enrichment factor of 55 after 60 minutes of extraction. A novel method of miniaturized voltammetric determination of HVA using cathodically pretreated boron doped diamond electrode was also developed.
Using differential pulse voltammetry, limit of detection, limit of quantificati was 1.9 mu mol dm(-3) on 6.6 mu mol dm(-3).