Spontaneously Hypertensive Rat Chromosome 2 with Mutant Connexin 50 Triggers Divergent Effects on Metabolic Syndrome Components
Abstract
Metabolic syndrome is a frequent condition with multifactorial aetiology. Previous studies indicated the presence of genetic determinants of metabolic syndrome components on rat chromosome 2 (RNO2) and syntenic regions of the human genome.
Our aim was to further explore these findings using novel rat models. We derived the BN-Dca and BN-Lx.
Dca congenic strains by introgression of a limited RNO2 region from a spontaneously hypertensive rat strain carrying a mutation in the Gja8 gene (SHR-Dca, dominant cataract) into the genomic background of Brown Norway strain and congenic strain BN-Lx, respectively. We compared morphometric, metabolic and cytokine profiles of adult male BN-Lx, BN-Dca and BN-Lx.
Dca rats. We performed in silico comparison of the DNA sequences throughout RNO2 differential segments captured in the new congenic strains.
Both BN-Dca and BN-Lx. Dca showed lower total triacylglycerols and cholesterol concentrations compared to BN-Lx.
Fasting insulin in BN-Dca was higher than in BN-Lx. Dca and BN-Lx.
Concentrations of several proinflammatory cytokines were elevated in the BN-Dca strain, including IL-1 alpha, IL-1 beta, IFN-gamma and MCP-1. In silico analyses revealed over 740 DNA variants between BN-Lx and SHR genomes within the differential segment of the congenic strains.
We derived new congenic models that prove that a limited genomic region of SHR-Dca RNO2 significantly affects lipid levels and insulin sensitivity in a divergent fashion.