Carborane-linked 2'-deoxyuridine 5'-O-triphosphate as building block for polymerase synthesis of carborane-modified DNA

Abstract

5-[(p-Carborane-2-yl) ethynyl]-2'-deoxyuridine 5'-O-triphosphate was synthesized and used as a good substrate in enzymatic construction of carborane-modified DNA or oligonucleotides containing up to 21 carborane moieties in primer extension reactions by DNA polymerases.

Keywords

• nucleotides
• nucleoside triphosphates
• carboranes
• DNA polymerase
• oligonucleotides