Abstract
This paper concerns the comparison of automatic volume estimation methods for isolated pancreatic islets. The estimated islet volumes are needed during the process of assessing the islet sample quality prior to the islet transplantation.
We study several different methods for automatic volume estimation. For this purpose we acquired a set of projections using optical tomography for a sample of an islet population.
Based on these projections we estimated the islet volumes using two stereological methods (the automatic Wulfsohn's method and the manual fakir method, considered to be the ground truth in this study), together with the filtered back projection followed by 3D segmentation. We have also employed two simple methods, currently used in medical practice, based on fitting a sphere or a prolate ellipsoid to a single binarized 2D islet projections.