Abstrakt
The capillary electrophoresis method has been developed for the analysis of phospholipid polar headgroups. The method consists in splitting the phospholipid samples by regioselective enzyme that cleaves the bond between polar headgroup and glycerol.
The presented capillary electrophroresis method operates in basic pH where all the phosphoesters are negatively charged and their mobilities are different enough to allow their separation. Separation electrolyte needs to be defined by ionic strength to give repeatable results.
Despite low absorbance of phosphoesters UV detection is more sensitive than the contactless conductometry one.