Voltammetric Study of DNA Nucleosides Labelled by Reducible Redox Markers at Boron-Doped Diamond Electrode
Abstract
Nitrophenyl triazole labelled deoxyadenosine (dA(TRNO2)) and deoxycytidine (dC(TRNO2)) were studied by cyclic voltammetry and square wave voltammetry in acetate buffer pH 5.0 at boron doped diamond electrode. Its flat inlaid disc enables mechanical polishing of the surface prior to each scan to ensure repeatable response.
Voltammetric signals corresponding to nitro group reduction and consequently the redox pair hydroxylamine/nitroso were well developed at potentials corresponding to the electrochemical behaviour of these compounds at other carbon-based materials. Strong adsorption of both labelled nucleosides at the electrode surface was observed.
Another redox label, benzofurazane, was attached to deoxycytidine (dC(BF)) nucleoside. The benzofurazane reduction signal was indistinct and the adsorption of the latter compound at the BDD surface was not observed.