Freezing processes in cryoprotective solutions of Me2SO, trehalose and antifreeze protein ApAFP752 and their impact on chromatin condition of cryopreserved cells
Abstract
In our work, we studied the behaviour of aqueous solutions of cryoprotective agents (Me2SO, trehalose, antifreeze protein ApAFP752) upon freezing/thawing. We characterized the course of phase change of all the investigated solutions at temperatures ranging from 20°C to -170°C using differential scanning calorimetry (shape and position of freezing/thawing curves) and other methods.