Characterization of KPC-Encoding Plasmids from Enterobacteriaceae Isolated in a Czech Hospital

Abstract

Ten Enterobacteriaceae isolates collected in a Czech hospital carried blaKPC-positive plasmids of different sizes (30, 45, and 80 kb). Sequencing revealed three types of plasmids (A to C) with the Tn4401a transposon.

Type A plasmids comprised an IncR backbone and a KPC-2-encoding multidrug resistance (MDR) region. Type B plasmids were derivatives of type A plasmids carrying an IncN3-like segment, while type C plasmids were IncP6 plasmids sharing the same KPC-2-encoding MDR region with type A and B plasmids.

Keywords

• Citrobacter freundii
• Tn4401a
• IncR
• ST18
• Illumina sequencing