Molecular sensor for orientative determination of epigenetic markers based on supramolecular polymers in aqueous physiological systems
Abstract
The principle of a molecular sensor technical solution for the orientative determination of epigenetic markers on the basis of supramolecular polymers in aqueous physiological systems is based on quantitative spectroscopic determination of UV / Vis or fluorescence. The principle is the interaction of methylated oligonucleotides with a chelate based on a saccharin derivative to form a supramolecular polymer in a buffered aqueous solution with a chrakterristic spectral change.
The functional sample consists, in particular, of a 5-fluorescence cuvette (1 cm long side of plastic). A small addition of about 25 μL of a concentrated chelator solution is added to each cuvette and 25 μL of a concentrated metal ion solution in water is added thereto.
After mixing the two solutions, a solution containing the sample is added and the cell volume is added to 2.4 ml. The sample is therefore analyzed analytically by five different supramolecular polymers.
Measured spectra are then compared to a library of measured standards. And the final evaluation is done by statistical methods.