Comparison of fluorometric and real-time PCR quantification of DNA extracted from formalin fixed tissue
2017
Abstract
The objective of this research was to compare the quantity of extracted DNA from liver tissue preserved in different solutions of formaldehyde with different durations of fixation and to decide which method is best suited for quantification. We compared the measurements of DNA concentration by fluorometric assays (BioSpectrometer (Eppendorf, Germany) in conjunction with the Qubit (R) dsDNA HS Assay Kit (Invitrogen, USA)) and qPCR-based assays (primers targeting sequences of ALU transposable elements with two different amplicon sizes of 63 bp and 229 bp).
Spectrophotometric methods were not applied due to their low sensitivity when measuring low DNA concentrations.