A more sensitive detection of micrometastases of NSCLC in lymph nodes using the one-step nucleic acid amplification (OSNA) method
Abstract
Detection of tumor cells in lymph nodes (LNs) removed during the Treatment of pulmonary tumor by radical surgery is limited by the possibilities of standart histopathological methods. The goal of this study was to obtain more accureate pTNM status by a more sensitive detection of micrometastases in LNs.
Results: identical results for H&E and OSNA examinations were recorded in 286 groups of LNs (89,4%). In total, positive examinations were recorded in 27 groups of LNs (8,4%) using the OSNA method, which were H&E negative.
In 7 groups of LNs (2,2%), the H&E examination was positive, while OSNA method produced negative results. Conclusions: the OSNA examination led to a higher pTNM stage classification in 14 (21,9%) patients.
The clinical significance remains the subject of follow-up research.