Abstrakt
Ultra-high performance liquid chromatography (UHPLC) has already become a mature and widely adopted separation approach across many application fields. High efficiency, high resolution, speed of analysis, robustness, reliability, and commercial availability of the wide range of UHPLC instruments and stationary phases based on sub-2-micron fully porous or sub-3-micron core-shell particles are important features attracting the attention of practitioners.
Coupling of UHPLC with mass spectrometry (MS) brings further advantages in terms of selectivity, sensitivity, and high-throughput for the analysis of complex samples. UHPLC coupled to triple quadrupole tandem MS or high-resolution MS is an important tool for both targeted and nontargeted analysis.
Within the past decade, UHPLC has established itself as an ideal fast-separation tool for the complex mixture analysis in both isocratic and gradient modes.